![]() The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. The addition of four barcodes produces over 3 million possible barcode combinations, allowing researchers to distinguish between both cells and samples.The Chromium Single Cell Gene Expression Solution Subsequent in-cell ligation reactions add two additional barcodes, with a fourth barcode added during the library stage. In-cell RT reactions use sample-specific barcodes to label each sample. Because the protocol is reagent based, there are fewer restrictions on cell size. Up to 100,000 cells from up to 48 samples can be barcoded in a single plate. Parse uses reagent-based barcoding for their highest throughput single cell option. Custom panels can be designed directly through MissionBio.ĭNA Panels | Mission Bio Parse Biosciences Each Tapestri chip can capture up to 10,000 cells. Tapestri panels are highly customizable for specific regions and genes of interest to minimize wasted sequence. The first and only platform for phenotypic and genotypic single cell analysis from the same cell, the Tapestri allows for barcoding of targeted DNA regions as well as protein identification. Protocol - TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 3.1 Protocol () Protocol - TotalSeq™-B or -C with 10x Feature Barcoding Technology () Visium spatial FFPE gene expression library prepĬell hashing, MULTIome, CSP barcode–5K reads/cellĪTAC – 25K reads/cell Recommended protocols:ĬG000053_CellPrepGuide_RevC.pdf ()ĬG000136_Demonstrated_Protocol_MethanolFixationCells_RevE.pdf ()ĬG000124_Demonstrated_Protocol_Nuclei_isolation_RevE.pdf ()ĬG000123_SamplePrepDemonstratedProtocol_-_CD3_Enrichment_RevB.pdf ()ĬG000147_Demonstrated_Protocol_Tumor_Dissociation_RevB.pdf ()ĬG00054_SamplePrepDemonstratedProtocol_-_CultCells_RevB.pdf ()ĬG000093_Demonstrated_Protocol_DeadCellRemoval_RevC.pdf ()ĬG00014_Demonstrated_Protocol_FreshFrozenHumanMouseCellLineMix_RevF_.pdf ()ĬG00039_Demonstrated_Protocol_FreshFrozenHumanPBMCs_RevD.pdf ()ĬG00055_Demonstrated_Protocol_Dissociation_Mouse_Neural_Tissue_Rev_C.pdf ()ĬG000149_DemonstratedProtocol_CellSurfaceProteinLabeling_RevC.pdf ()ĬG000233_Demonstrated_Protocol_Thawing_Dissociated_TumorCells_Rev_A.pdf () CG000391_CellLabelingwithCellMultiplexingOligo_RevA.pdf () Visium spatial gene expression library prep Single cell multiome with ATAC & 3’ gene expression with MULTIome Single cell multiome with ATAC & 3’ gene expression Servicesģ’ gene expression with cell surface protein expressionģ’ gene expression multiplexing with cell hashing or multiomeĥ’ gene expression with cell surface protein expression ![]() Probe based assays are available for human and mouse. The CytAssist will enable the use of pre-cut FFPE slides for spatial transcriptomics on the 10X Genomics Visium FFPE platform. The latest release from 10X Genomics for single cell encapsulation, the Chromium X allows for capture of up to 20,000 cells per lane, with even higher numbers for fixed cells. Methods are available for 3’ and 5’ library prep as well as 3’ fixed cells from human and mouse. This new platform combines the well established 10X methodology with new higher throughput methods to increase cell target number and decreasing doublet rates. The protocol is available for 3’ and 5’ expression, immune profiling, ATAC and spatial transcriptomics. Subsequent library prep barcodes individual samples to pool for sequencing. The RT microreaction adds the cellular barcode, and GEMS are ruptured, creating a pool of barcoded cDNA. Cells, RT reagents and barcoded gel beads are mixed to create gel beads in emulsion (GEMs), isolating individual cells with a unique primer. The 10X Chromium Controller processes up to 10,000 cells per lane. ![]() We offer three different platforms with the ability to sequence RNA, DNA and protein on a single cell level. The Single Cell & Transcriptomics Core has been on the cutting edge of single cell research at Johns Hopkins since 2017.
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